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KMID : 0903519990420030193
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.193 ~ p.198
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Optimization of Culture Conditions of Chitosanase - producing Bacillus sp. P16
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Jung Mi-Ra
Park Ro-Dong
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Abstract
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The optimal culture condition of Bacillus sp. P16 was investigated for production of an extracellular endo-splitting chitosanase. The best carbon and nitrogen sources for the chitosanase production were chitosan and tryptone, respectively. The best condition for the maximum activity was at 37℃ in a medium containing 0.5% powdered chitosan, 1% tryptone, and 1% NaCl(at initial pH 7.0) in a rotary shaker(200 rpm). In a jar fermenter, the culture duration shortened to 6∼12 hr for maximum activity and the enzyme activity increased about 100% compared with that of flask culture.
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